文獻來源：更新的 AVMA 動物安樂死指南：2020 版
- 齧齒類動物以二氧化碳(CO2)安樂死政策更新
(二氧化碳氣體置換率由每分鐘10-30%提高到30-70%)
Animals must be placed in uncharged chambers and flow rates must be used which displace 30-70% of the chamber volume per minute. (For a 10-liter volume chamber, use a flow rate of approximately 3-7 liter(s) per minute.) After the animals become unconscious, the flow rate can be increased to minimize the time to death. o Displacement rate is critical to the humane application of CO2, an appropriate pressure-reducing regulator and flow meter or equivalent equipment with demonstrated capability for generating the recommended displacement rates for the size container being utilized is absolutely necessary.
必須將動物放置在不帶電的腔室中，並且必須使用每分鐘置換30-70%腔室體積的流速。（對於10升容積室，請使用大約每分鐘3-7升的流速。動物失去知覺後，可以增加流速以盡量減少死亡時間。o 置換率對於二氧化碳的人道應用至關重要，絕對需要適當的減壓調節器和流量計或同等設備，這些設備具有為所用容器尺寸產生推薦排水率的能力。